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Membrane/transmembrane protein overexpression cell lines
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HEK293, CHO,Jurkat, MC38, THP-1, U937,LLC, Raji, AGS,NIH3T3,SNU16 and other
cells
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1. Validation of target overexpression at gene and protein levels
2. Positive rate of target protein ＞ 70%
3. Cell line cryopreservation density:
5 × 10^6 cells/vial
4. No bacteria and mycoplasma contamination
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4-6 weeks
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Luciferase reporter system
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Jurkat-NFAT,HEK293-NFAT,and other customized target types
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1. Lentiviral infection
2. Electroporation
3. Chemical reagent transfection
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Puromycin
Hygromycin
G418
MSX (GS system)
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1. Validation of target overexpression at gene and protein levels
2. Luciferase signal value ＞ 10,000
2. Positive rate of target protein ＞ 70%
3. Cell line cryopreservation density: 5 × 10^6 cells/vial
4. No bacteria and mycoplasma contamination
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4-6 weeks
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Secreted antigen/antibody overexpression cell lines
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CHO-S,
CHO-K1S,
CHOZN,
CHO-K1Q
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1. Deliver mg-level protein samples
2. Provided secretory cell expression level ＞ 0.5 g/L
3. Cell line cryopreservation density: 5 × 10^6 cells/vial
4. No bacteria and mycoplasma contamination
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6-8 weeks
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